I don't know that any of those 3 are safe bets to make the tournament. I don't follow other teams that closely, so I don't know what Wisconsin has coming up to replace Hayes and Koening. But, given their track record, I would say Wisconsin is the likeliest of the three to make the tournament.
I, personally, think Iowa should be better defensively (could they get much worse?) and more balanced on offense next season. It's certainly possible that Iowa will be a bubble team again next year, depending on how well the younger guys develop and continue to get better. In truth, Iowa surpassed my expectations in the 2nd half of the season, and it's certainly possible they will again next season. If they surpass expectations, they will move from the bubble to a team that gets an at large bid.
Iowa State is a total mystery. Obviously, they lose a lot (4 starters). On paper, the guys they have coming in aren't exactly chopped liver, though. But I'm sure Iowa State will have a typical ebb and flow learning curve like any other program who's had a significant youth movement. In short, conventional wisdom would suggest they will be wildly inconsistent throughout the year, and on the outside looking in come March.
It's May, though. A lot can happen in 10 months (injuries, unforeseen player growth or regression, etc.)